ABSTRAK

LatarBelakang: Penyakit infeksi masih merupakan penyebab utama morbiditas dan mortalitas di dunia dan dapat menyebabkan bermacam-macam infeksi seperti pneumonia, meningitis, endokarditis. Di samping itu penyakit infeksi juga bertanggung jawab pada penurunan kualitas hidup jutaan penduduk di berbagai negara maju dan berkembang. Menurut WHO sebanyak 25 juta kematian diseluruh dunia pada tahun 2011, sepertiganya disebabkan oleh penyakit infeksi. Infeksi bakteri Staphylococcus aureus dapat ditemukan pada permukaan kulit sebagai flora normal, terutama disekitar hidung, mulut, alat kelamin,dan anus Tumbuhan roviga (Calotropis gigantea) merupakan salah satu tumbuhan yang memiliki kandungan bermanfaat sebagai obat, tetapi masih kurang dimanfaatkan oleh masyarakat. Daun roviga memiliki efek antibakterial, antifertilitas, analgesik, anti-inflamasi, antipiretik, ekspektoran, dan untuk penyakit kulit. Zat-zat yang terkandung dalam tanaman roviga seperti tanin dan flavonoid..
Tujuan: Untuk mengetahui daya hambat ekstrak daun roviga terhadap pertumbuhan Staphylococcus aureus
Metode: Jenis desain penelitian yang digunakan pada penelitian ini yaitu desain penelitian true experimental post test control group design. Pengumpulan sampel yang dibutuhkan, pembuatan ekstrak dengan beberapa konsentrasi, pembuatan media yang dibutuhkan agar bakteri dapat hidup, pemberian ekstrak pada bakteri serta penentuan kadar hambat minimal. didapatkan jumlah sampel untuk tiap kelompok sebanyak ≥ 4 sampel. Peneliti melakukan replikasi sebanyak 6 kali sehingga jika dikalikan dengan 6 kelompok perlakuan didapatkan besaran sampel yang akan diuji berjumlah 36 sampel.
Hasil: pada konsentrasi 10% rerata 0, konsentrasi 25% rerata 0, pada konsentrasi 50% rerata 11,3, dan pada konsentrasi 100% rerata 15,4, kontrol positif (+) rerata 32,2, kontrol negatif (-) rerata 0.
Kesimpulan: Ekstrak daun roviga memiliki daya hambat terhadap bakteri Staphylococcus aureus
Kata kunci: daun roviga (Calotropis gigantea), Staphylococcus aureus.
INHIBITORY TEST OF ROVIGA LEAVE’S (Calotropis gigantea) EXTRACT AGAINST STAPHYLOCOCCUS AUREUS BACTERIA
Afanny Bijak Pawindu*, Try Setyawati**, Christin Rony Nayoan***

*Medical Student, Faculty of Medicine and Health Science, Tadulako University
**Department of Biochemistry, Faculty of Medicine and Health Science, Tadulako University
***Department of Farmacology, Faculty of Medicine and Health Science, Tadulako University

ABSTRACT

Background: Infectious diseases are still the leading cause of morbidity and mortality in the world and can cause various infections such as pneumonia, meningitis, endocarditis. In addition, infectious diseases are also responsible for the decline of the quality of life of millions of people in various developed and developing countries. According to WHO as many as 25 million deaths worldwide in 2011, one-third caused by infectious diseases. Staphylococcus aureus bacterial infections can be found on the surface of the skin as a normal flora, especially around the nose, mouth, genitals, and anus. Roviga plant (Calotropis gigantea) is one of the plants that have a useful content as a drug, but still underutilized by the community. Leaf roviga has antibacterial, antifertility, analgesic, anti-inflammatory, antipyretic, expectorant, and skin diseases effects. Substances contained in roviga plants such as tannins and flavonoids.
Objective: To know the inhibition power of roviga leaf extract on growth of Staphylococcus aureus.
Methods: Type of research design used in this research is true experimental design post test control group design. Collection of required samples, making extracts with some concentration, making the media needed for bacteria to live, giving extracts on bacteria and determination of minimal inhibitory levels. We get the number of samples for each group as many as ≥ 4 samples. Researcher replicate 6 times so that if multiplied by 6 treatment group obtained sample size to be tested amounted 36 sample.
Results: Average are 0 of 10% concentration, 0 average of 25% concentration , 11,3 average of 50% concentration and 15,4 average of 100% concentration, 32,2 average of positive control, 0 average of negative control.
Conclusion: Roviga leaf extract has a resistance to Staphylococcus aureus bacteria

Keywords: Roviga’s Leaves (Calotropis gigantea), Staphylococcus aureus